LOCUS       NEU3-pGEc2              6609 bp    DNA     circular     21-SEP-2016
FEATURES             Location/Qualifiers
     misc_feature    complement(6087..6216)
                     /note="3'-ITR"
     misc_feature    complement(5785..5999)
                     /note="BGH pA"
     rep_origin      1137..1804
                     /dnas_title="Col E1 origin"
                     /label=Col E1 origin
                     /vntifkey="33"
     CDS             129..989
                     /dnas_title="AmpR"
                     /label=AmpR
                     /vntifkey="4"
     misc_feature    1998..2127
                     /note="5'ITR"
     misc_feature    2208..2790
                     /note="CMV Promoter"
     misc_feature    2828..2960
                     /note="Artificial Intron"
     misc_feature    5237..5778
                     /note="WPRE"
     misc_recomb     complement(2974..3001)
                     /label=attB1
                     /note="attB1 site"
                     /note="recognized as attB1"
     misc_feature    4416..4438
                     /note="attB2"
     misc_feature    5216..5221
                     /note="BstB1 site"
     misc_feature    4439..5134
                     /note="Super GFP"
     misc_feature    5135..5185
                     /note="AviTag"
     misc_feature    5186..5209
                     /note="His Tag"
     PCR_frame       3011..4415
                     /note="Reading Frame"
                     /set="Set 1"
     misc_feature    3011..4393
                     /note="OPTIMIZED NEU3 coding region"
     misc_feature    4394..4415
                     /note="TEV"
     vector          join(4424..6609,1..2991)
                     /source="pGEc2-DEST"
                     /type="Destination Vector"
     clone           2992..4423
                     /source="NEU3 pDONR"
                     /type="Entry Clone"
     source          1..6609
                     /dnas_title="NEU3-pGEc2"
ORIGIN      
        1 GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT
       61 CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA
      121 GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT
      181 GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT
      241 TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT
      301 TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG
      361 TATTATCCCG TATTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA
      421 ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA
      481 GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA
      541 CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA
      601 CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA
      661 CCACGATGCC TGTAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA
      721 CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC
      781 TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC
      841 GTGGTTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG
      901 TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA
      961 TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT
     1021 AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA
     1081 ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG
     1141 AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA
     1201 CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT
     1261 TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTTCTT CTAGTGTAGC
     1321 CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA
     1381 TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA
     1441 GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC
     1501 CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA
     1561 GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA
     1621 CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG
     1681 GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC
     1741 TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG
     1801 CTCATTAGGC ACCCCAGGCT TTACCCGAAC GACCGAGCGC AGCGAGTCAG TGAGCGAGGA
     1861 AGCGGAGAGC GCCCAATACG CAAGGAAACA GCTATGACCA TGTTAATGCA GCTGGCACGA
     1921 CAGGTTTCCC GACTGGAAAG CGGGCAGTGA GCGGAAGGCC CATGAGGCCA GTTAATTAAG
     1981 AGGTACCTTA ATTAAGGCTG CGCGCTCGCT CGCTCACTGA GGCCGCCCGG GCAAAGCCCG
     2041 GGCGTCGGGC GACCTTTGGT CGCCCGGCCT CAGTGAGCGA GCGAGCGCGC AGAGAGGGAG
     2101 TGGCCAACTC CATCACTAGG GGTTCCTTGT AGTTAATGAT TAACCCGCCA TGCTACTTAT
     2161 CTACGTAGCC ATGCTCTAGG AAGATCGCAA TTCGCCCTTA AGCTAGCTAG TTATTAATAG
     2221 TAATCAATTA CGGGGTCATT AGTTCATAGC CCATATATGG AGTTCCGCGT TACATAACTT
     2281 ACGGTAAATG GCCCGCCTGG CTGACCGCCC AACGACCCCC GCCCATTGAC GTCAATAATG
     2341 ACGTATGTTC CCATAGTAAC GCCAATAGGG ACTTTCCATT GACGTCAATG GGTGGAGTAT
     2401 TTACGGTAAA CTGCCCACTT GGCAGTACAT CAAGTGTATC ATATGCCAAG TACGCCCCCT
     2461 ATTGACGTCA ATGACGGTAA ATGGCCCGCC TGGCATTATG CCCAGTACAT GACCTTATGG
     2521 GACTTTCCTA CTTGGCAGTA CATCTACGTA TTAGTCATCG CTATTACCAT GGTGATGCGG
     2581 TTTTGGCAGT ACATCAATGG GCGTGGATAG CGGTTTGACT CACGGGGATT TCCAAGTCTC
     2641 CACCCCATTG ACGTCAATGG GAGTTTGTTT TGGCACCAAA ATCAACGGGA CTTTCCAAAA
     2701 TGTCGTAACA ACTCCGCCCC ATTGACGCAA ATGGGCGGTA GGCGTGTACG GTGGGAGGTC
     2761 TATATAAGCA GAGCTGGTTT AGTGAACCGT CAGATCCTGC AGAAGTTGGT CGTGAGGCAC
     2821 TGGGCAGGTA AGTATCAAGG TTACAAGACA GGTTTAAGGA GACCAATAGA AACTGGGCTT
     2881 GTCGAGACAG AGAAGACTCT TGCGTTTCTG ATAGGCACCT ATTGGTCTTA CTGACATCCA
     2941 CTTTGCCTTT CTCTCCACAG GTGTCCAGCG GCCATCACAA GTTTGTACAA AAAAGCAGGC
     3001 TCGCGCCACC ATGAGGCCTG CTGATCTGCC TCCTAGACCC ATGGAAGAGA GCCCTGCTTC
     3061 TAGCAGCGCC CCTACAGAGA CAGAGGAACC TGGAAGCTCC GCCGAAGTGA TGGAAGAAGT
     3121 GACCACCTGT AGCTTCAACA GCCCCCTGTT CCGGCAGGAA GATGACAGAG GCATCACCTA
     3181 CAGAATCCCC GCCCTGCTGT ATATCCCCCC CACACATACT TTTCTGGCCT TCGCCGAGAA
     3241 GCGGAGCACC AGACGAGATG AAGATGCTCT GCACCTGGTG CTGCGGAGAG GACTGAGAAT
     3301 TGGACAGCTG GTGCAGTGGG GACCCCTGAA ACCTCTCATG GAAGCCACAC TGCCTGGCCA
     3361 CAGAACCATG AATCCTTGCC CCGTGTGGGA GCAGAAAAGC GGCTGCGTGT TCCTGTTCTT
     3421 TATCTGCGTG CGGGGCCACG TGACCGAGAG ACAGCAGATC GTGTCTGGCA GAAATGCCGC
     3481 CAGACTGTGC TTCATCTATA GCCAGGATGC CGGCTGCTCT TGGAGCGAAG TGCGGGATCT
     3541 GACCGAGGAA GTGATCGGCT CTGAACTGAA GCACTGGGCC ACATTTGCCG TGGGACCTGG
     3601 ACATGGAATT CAGCTGCAGA GCGGCAGACT GGTCATCCCT GCCTACACCT ACTACATCCC
     3661 CAGCTGGTTC TTTTGTTTTC AGCTCCCCTG CAAGACCAGA CCCCACAGCC TGATGATCTA
     3721 CAGCGACGAT CTGGGCGTGA CATGGCACCA CGGAAGGCTG ATCAGACCTA TGGTCACCGT
     3781 GGAATGCGAG GTGGCCGAAG TCACAGGCAG AGCAGGACAT CCTGTGCTGT ACTGTAGCGC
     3841 CAGAACCCCC AACAGATGTA GAGCCGAGGC CCTGTCTACA GATCACGGCG AGGGATTTCA
     3901 GAGACTGGCC CTCAGCAGAC AGCTGTGTGA ACCTCCACAT GGCTGTCAGG GCAGCGTGGT
     3961 GTCCTTCAGA CCTCTGGAAA TTCCCCACAG ATGCCAGGAC AGCAGCAGCA AGGATGCCCC
     4021 AACAATCCAG CAGTCTAGCC CTGGCAGCAG CCTGAGACTC GAGGAAGAAG CTGGCACACC
     4081 TTCTGAGAGC TGGCTCCTGT ACAGCCACCC TACCAGCAGA AAGCAGAGAG TGGACCTGGG
     4141 CATCTACCTG AATCAGACAC CACTGGAAGC CGCCTGTTGG AGCAGACCTT GGATTCTGCA
     4201 CTGTGGCCCT TGCGGCTATT CTGATCTGGC AGCCCTGGAA GAGGAAGGCC TGTTCGGCTG
     4261 TCTGTTCGAG TGTGGCACCA AGCAGGAATG TGAACAGATC GCCTTTAGAC TGTTCACCCA
     4321 CAGAGAGATC CTGAGCCATC TGCAGGGCGA TTGCACAAGC CCCGGAAGAA ACCCTAGCCA
     4381 GTTCAAGAGC AACgaaaact tgtactttca aggctCCAGC TTTCTTGTAC AAAGTGGTAT
     4441 GAGCAAGGGC GAGGAACTGT TCACCGGCGT GGTGCCCATC CTGGTGGAAC TGGATGGCGA
     4501 CGTGAACGGC CACAAGTTCT CTGTGCGGGG AGAGGGCGAA GGCGACGCCA CAAATGGCAA
     4561 GCTGACCCTG AAGTTCATCT GCACCACCGG CAAGCTGCCC GTGCCTTGGC CTACCCTCGT
     4621 GACCACACTG ACCTACGGCG TGCAGTGCTT CAGCAGATAC CCCGACCATA TGAAGCGGCA
     4681 CGACTTCTTC AAGAGCGCCA TGCCCGAGGG CTACGTGCAG GAACGGACCA TCAGCTTCAA
     4741 GGACGACGGC ACCTACAAGA CCAGAGCCGA AGTGAAGTTC GAGGGCGACA CCCTCGTGAA
     4801 CCGGATCGAG CTGAAGGGCA TCGATTTCAA AGAGGACGGC AACATCCTGG GCCACAAGCT
     4861 GGAGTACAAC TTCAACAGCC ACAACGTGTA CATCACCGCC GACAAGCAGA AGAACGGCAT
     4921 CAAGGCCAAC TTCAAGATCC GGCACAACGT GGAAGATGGC AGCGTGCAGC TGGCCGACCA
     4981 CTACCAGCAG AACACCCCCA TCGGAGATGG CCCCGTGCTG CTGCCCGACA ACCACTACCT
     5041 GAGCACCCAG AGCGTGCTGA GCAAGGACCC CAACGAGAAG CGGGACCACA TGGTGCTGCT
     5101 GGAATTCGTG ACCGCCGCTG GCATCACCCA CGGCATGAGC GGCCTGAACG ACATCTTCGA
     5161 GGCCCAGAAA ATCGAGTGGC ACGAGCACCA CCATCATCAC CATCACCACT GATGATTCGA
     5221 AAAATAATAA GGATCCAATC AACCTCTGGA TTACAAAATT TGTGAAAGAT TGACTGGTAT
     5281 TCTTAACTAT GTTGCTCCTT TTACGCTATG TGGATACGCT GCTTTAATGC CTTTGTATCA
     5341 TGCTATTGCT TCCCGTATGG CTTTCATTTT CTCCTCCTTG TATAAATCCT GGTTGCTGTC
     5401 TCTTTATGAG GAGTTGTGGC CCGTTGTCAG GCAACGTGGC GTGGTGTGCA CTGTGTTTGC
     5461 TGACGCAACC CCCACTGGTT GGGGCATTGC CACCACCTGT CAGCTCCTTT CCGGGACTTT
     5521 CGCTTTCCCC CTCCCTATTG CCACGGCGGA ACTCATCGCC GCCTGCCTTG CCCGCTGCTG
     5581 GACAGGGGCT CGGCTGTTGG GCACTGACAA TTCCGTGGTG TTGTCGGGGA AGCTGACGTC
     5641 CTTTCCATGG CTGCTCGCCT GTGTTGCCAC CTGGATTCTG CGCGGGACGT CCTTCTGCTA
     5701 CGTCCCTTCG GCCCTCAATC CAGCGGACCT TCCTTCCCGC GGCCTGCTGC CGGCTCTGCG
     5761 GCCTCTTCCG CGTCTTCGAG ATCTGCCTCG ACTGTGCCTT CTAGTTGCCA GCCATCTGTT
     5821 GTTTGCCCCT CCCCCGTGCC TTCCTTGACC CTGGAAGGTG CCACTCCCAC TGTCCTTTCC
     5881 TAATAAAATG AGGAAATTGC ATCGCATTGT CTGAGTAGGT GTCATTCTAT TCTGGGGGGT
     5941 GGGGTGGGGC AGGACAGCAA GGGGGAGGAT TGGGAAGACA ATAGCAGGCA TGCTGGGGAC
     6001 TCGAGTTAAG GGCGAATTGC CGATTAGGAT CTTCCTAGAG CATGGCTACG TAGATAAGTA
     6061 GCATGGCGGG TTAATCATTA ACTACAAGGA ACCCCTAGTG ATGGAGTTGG CCACTCCCTC
     6121 TCTGCGCGCT CGCTCGCTCA CTGAGGCCGG GCGACCAAAG GTCGCCCGAC GCCCGGGCTT
     6181 TGCCCGGGCG GCCTCAGTGA GCGAGCGAGC GCGCAGCCTT AATTAAGAGC TCATGGCGCG
     6241 CCTAGGCCTT GACGGCCTTC CGCCAATTCG CCCTATAGTG AGTCGTATTA CGTCGCGCTC
     6301 ACTGGCCGTC GTTTTACAAC GTCGTGACTG GGAAAACCCT GGCGTTACCC AACTTAATCG
     6361 CCTTGCAGCA CATCCCCCTT TCGCCAGCTG GCGTAATAGC GAAGAGGCCC GCACCGAAAC
     6421 GCCCTTCCCA ACAGTTGCGC AGCCTGAATG GCGAATGGGA GCGCCCTGTA GCGGCCACTC
     6481 AACCCTATCT CGGTCTATTC TTTTGATTTA TAAGGGATTT TGCCGATTTC GGCCTATTGG
     6541 TTAAAAAATG AGCTGATTTA ACAAAAATTT AACGCGAATT TTAACAAAAT ATTAACGCTT
     6601 ACAATTTAG
//

